Human FOXP3 ELISA Kit

Key features and details

Sensitivity: 0.188 ng/ml
Range: 0.313 ng/ml – 20 ng/ml
Sample type: Plasma, Serum, Tissue Homogenate
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

Overview

Product name
Human FOXP3 ELISA Kit
See all FOXP3 kits
Detection method
Colorimetric
Sample type
Serum, Plasma, Tissue Homogenate
Assay type
Sandwich (quantitative)
Sensitivity
< 0.188 ng/ml
Range
0.313 ng/ml – 20 ng/ml
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
This FOXP3 ELISA kit (ab285290, K4224) is a sandwich ELISA assay for the quantitative measurement of human FOXP3 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of FOXP3 captured from the samples.
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K4224 FOXP3 (Human) ELISA Kit. K4224-100 is the same size as the 96 test size of ab285290.
Platform
Microplate (12 x 8 well strips)

Properties

Storage instructions
Store at +4°C. Please refer to protocols.

Components	96 tests
25X Wash buffer	1 x 30ml
Antibody dilution buffer	1 x 10ml
Biotin-Detection antibody (Concentrated)	1 x 120µl
HRP-Streptavidin Conjugate (SABC)	1 x 120µl
Lyophilized Standard	2 vials
Micro ELISA Plate	1 unit
Plate Sealer	5 units
SABC dilution buffer	1 x 10ml
Sample and Standard Dilution Buffer	1 x 20ml
Stop Solution	1 x 10ml
TMB Substrate	1 x 10ml

Research areas
- Cell Biology
- Cell Cycle
- Cell Division
- Chromatid Cohesion
- Epigenetics and Nuclear Signaling
- Transcription
- Domain Families
- Forkhead Box
- FOXP
- Immunology
- Adaptive Immunity
- Regulatory T Cells
Function
Probable transcription factor. Plays a critical role in the control of immune response.
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accession Q9BZS1The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .
Information by UniProt
Alternative names
- AIID
- DIETER
- Forkhead box P3
- Forkhead box protein P3
- FOXP3
- FOXP3_HUMAN
- FOXP3delta7
- Immune dysregulation polyendocrinopathy enteropathy X linked
- Immunodeficiency polyendocrinopathy enteropathy X linked
- IPEX
- JM2
- MGC141961
- MGC141963
- OTTHUMP00000025832
- OTTHUMP00000025833
- OTTHUMP00000226737
- PIDX
- Scurfin
- XPID
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Database links
- Entrez Gene: 50943 Human
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- Unigene: 247700 Human

Associated products

Alternative Versions
- FITC Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (ab179898)
- Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] – Azide free (ab179914)
Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
- Goat Anti-Mouse IgM H&L (ab9167)
- Rabbit Anti-Mouse IgM mu chain (ab9175)