Anti-5-methylcytosine (5-mC) antibody [5MC-CD]

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Key features and details

  • Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC)
  • Suitable for: Southern Blot, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgM

Overview

  • Product name

    Anti-5-methylcytosine (5-mC) antibody [5MC-CD]
    See all 5-methylcytosine (5-mC) primary antibodies
  • Description

    Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC)
  • Host species

    Mouse
  • Specificity

    ab73938 reacts with DNA with 5-Methylcytosine (methylated DNA).
  • Tested applications

    Suitable for: Southern Blot, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC) conjugated to Bovine Serum Albumin (BSA).

  • Positive control

    • Chlamidomonas me-1 cells.
  • General notes

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 6
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration
    • 100 µg at 1 mg/ml
    • Purification notes

      ab73938 is purified and filter-sterilised.
    • Clonality

      Monoclonal
    • Clone number

      5MC-CD
    • Isotype

      IgM

      Research areas

      • Epigenetics and Nuclear Signaling
      •  
      • DNA methylation
      •  
      • Methylated DNA
      • Epigenetics and Nuclear Signaling
      •  
      • DNA methylation
      •  
      • DNMTs
      • Epigenetics and Nuclear Signaling
      •  
      • DNA / RNA
      •  
      • DNA / Nucleotides

Associated products

  • Alternative Versions

    • FITC Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (ab179898)
    • Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] – Azide free (ab179914)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab73938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

  • Form

    The native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application.
  • Alternative names

    • 5 m C antibody
    • 5 mC antibody
    • 5 me C antibody
    • 5 Me Cytidine antibody
    • 5 Me Cytosine antibody
    • 5 meC antibody
    • 5 Methycytosine antibody
    • 5 Methyl Cytidine antibody
    • 5 Methyl Cytosine antibody
    • 5 Methyl-Cytidine antibody
    • 5 Methyl-Cytosine antibody
    • 5 MethylCytidine antibody
    • 5 MethylCytosine antibody
    • 5-mC antibody
    • 5-Me Cytidine antibody
    • 5-Me Cytosine antibody
    • 5-meC antibody
    • 5-Methyl Cytidine antibody
    • 5-Methyl-Cytidine antibody
    • 5-Methyl-Cytosine antibody
    • 5-MethylCytidine antibody
    • 5-MethylCytosine antibody
    • 5mC antibody
    • methyl CpG antibody

Protocols