Histamine ELISA Kit

Key features and details

Sensitivity: 0.938 ng/ml
Range: 1.563 ng/ml – 100 ng/ml
Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Homogenate
Detection method: Colorimetric
Assay type: Competitive

Overview

Product name

Histamine ELISA Kit

Detection method

Colorimetric

Sample type

Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Homogenate

Assay type

Competitive

Sensitivity

< 0.938 ng/ml

Range

1.563 ng/ml – 100 ng/ml

Assay duration

Multiple steps standard assay

Product overview

This ELISA kit is used for in vitro quantitative determination of Histamine.

Species reactivity: Universal.

Histamine is an organic nitrogenous compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine is involved in the inflammatory response and has a central role as a mediator of pruritus. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.

This Histamine ELISA kit (ab285333, K4163) uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with histamine. During the reaction, histamine in the sample or standard competes with a fixed amount of histamine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to histamine. The concentration of histamine in the samples is then determined by comparing the O.D. of the samples to the standard curve.