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Human IL-12 p40 + IL-12 p70 ELISA Kit

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Key features and details

  • Sensitivity: 20 pg/ml
  • Range: 62.5 pg/ml – 2000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-12 p40 + IL-12 p70 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    SamplenMeanSDCV%
    Overall   3.7%
    Inter-assay
    SamplenMeanSDCV%
    Sample 1182277pg/ml1607%
    Sample 2181199pg/ml1089%
    Sample 318479pg/ml4910.3%
    Sample 4181488pg/ml1238.3%
    Sample 518826pg/ml10612.8%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 20 pg/ml

  • Range

    62.5 pg/ml – 2000 pg/ml

  • Recovery

    100 %

  • Assay time

    1h 45m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s IL-12 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 in Human serum, plasma, buffered solutions and supernatants.


    A monoclonal antibody specific for IL-12 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 present in the samples. 


    This kit will recognize both endogenous and recombinant Human IL-12, and the Human Il-12 p40 monomer, dimer or heterodimer p70 (p40 + p35) forms of the molecule.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • ComponentsIdentifier1 x 96 tests2 x 96 tests
    10X Standard Diluent BufferBlack1 x 15ml1 x 25ml
    200X Wash BufferWhite1 x 10ml2 x 10ml
    Biotinylated Antibody DiluentRed1 x 7ml1 x 13ml
    Biotinylated anti-IL12Red1 x 0.4ml2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml1 x 24ml
    HRP DiluentRed1 x 12ml1 x 23ml
    IL-12 ControlSilver2 vials4 vials
    IL-12 Microplate (12 x 8 well strips) 1 unit2 units
    IL-12 Standard (Lyophilized)Yellow2 vials4 vials
    Plastic Plate Covers 2 units4 units
    Standard Diluent (Serum) 1 x 7ml2 x 7ml
    Stop ReagentBlack1 x 11ml2 x 11ml
    Streptavidin-HRP 2 x 5µl4 x 5µl

  • Research areas

    • Immunology
      •  
    • Innate Immunity
      •  
    • Macrophage / Inflamm.
    • Immunology
      •  
    • Innate Immunity
      •  
    • Cytokines
      •  
    • Interleukins
    • Cancer
      •  
    • Tumor immunology
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    • Cytokines
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    • Interleukins
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  • Function

    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
    Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

  • Involvement in disease

    Mendelian susceptibility to mycobacterial disease
    Psoriasis 11

  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.

  • Cellular localization

    Secreted.

Protocols

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