Human TACE ELISA Kit (ADAM17)

Key features and details

Product name
Human TACE ELISA Kit (ADAM17)
Detection method
Colorimetric
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)
Sensitivity
< 70 pg/ml
Range
78.15 pg/ml – 5000 pg/ml
Recovery
100 %
Sample specific recovery
Sample type Average % Range
Cell culture supernatant 98.65 89% – 124%
Serum 112.3 85% – 132%
Plasma 101.1 72% – 128%
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s TACE Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TACE in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human TACE coated on a 96-well plate. Standards and samples are pipetted into the wells and TACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TACE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Notes
Optimization may be required with urine samples.
The standard is affinity purified.
Platform
Microplate

Components	Identifier	1 x 96 tests
200X HRP-Streptavidin Concentrate		1 x 200µl
20X Wash Buffer		1 x 25ml
5X Assay Diluent B		1 x 15ml
Assay Diluent C		1 x 30ml
Biotinylated anti-Human TACE	Item F	2 vials
Recombinant Human TACE Standard (lyophilized)		2 vials
Stop Solution		1 x 8ml
TACE Microplate (12 x 8 wells)	Item A	1 unit
TMB One-Step Substrate Reagent		1 x 12ml