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Human Interferon gamma ELISA Kit (IFNG)

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Key features and details

  • Sensitivity: 15 pg/ml
  • Range: 20.6 pg/ml – 15000 pg/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Interferon gamma ELISA Kit (IFNG)
    See all Interferon gamma kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 15 pg/ml

  • Range

    20.6 pg/ml – 15000 pg/ml

  • Recovery

    92 %

    Sample specific recovery
    Sample typeAverage %Range
    Cell culture extracts92.2582% – 103%
    Tissue Extracts91.7380% – 101%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s Interferon gamma (IFNG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Interferon gamma in cell lysates and tissue lysates.


    This assay employs an antibody specific for Human Interferon gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.

  • Notes

     

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components1 x 96 tests
    20X Wash Buffer1 x 25ml
    2X Cell Lysis Buffer1 x 5ml
    400X HRP-Streptavidin Concentrate1 x 200µl
    5X Assay Diluent1 x 15ml
    5X Sample Diluent Buffer1 x 10ml
    Biotinylated anti-Human Interferon gamma2 vials
    Interferon gamma Microplate (12 x 8 wells)1 unit
    Recombinant Human Interferon gamma Standard (lyophilized)2 vials
    Stop Solution1 x 8ml
    TMB One-Step Substrate Reagent1 x 12ml

  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Interferon gamma
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interferons
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Neuroscience
    • Processes

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

  • Tissue specificity

    Released primarily from activated T lymphocytes.

  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.

  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

  • Cellular localization

    Secreted.

Protocols

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