Human Anti-Toxocara canis IgG ELISA Kit

Key features and details

Sample type: Cit plasma, Hep Plasma, Serum
Detection method: Colorimetric
Assay type: Indirect
Reacts with: Human

Overview

Product name

Human Anti-Toxocara canis IgG ELISA Kit

Detection method

Colorimetric

Precision

Intra-assay
Sample	n	Mean	SD	CV%
Pos Serum	12			2.6%

Inter-assay
Sample	n	Mean	SD	CV%
Pos. Serum	5			8.1%

Sample type

Serum, Hep Plasma, Cit plasma

Assay type

Indirect

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Human

Product overview

Abcam’s anti-Toxocara canis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Toxocara canis in Human serum and plasma.

A 96-well plate has been precoated with Toxocara canis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Toxocara canis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxocara canis IgG sample captured in plate.

Platform

Microplate

Properties

Storage instructions

Store at +4°C. Please refer to protocols.

Components	Identifier	1 x 96 tests
20X Washing Solution	White cap	1 x 50ml
Cover foil		1 unit
IgG Sample Diluent	Yellow, White cap	1 x 100ml
Stop Solution	red cap	1 x 15ml
Strip holder		1 unit
TMB Substrate Solution	Yellow cap	1 x 15ml
Toxocara canis Coated Microplate (12 x 8 wells)	12 strips of 8 wells	1 unit
Toxocara canis Cut-off Control	Yellow, Green cap	1 x 3ml
Toxocara canis Negative Control	Yellow, Blue cap	1 x 2ml
Toxocara canis Positive Control	Yellow, Red cap	1 x 2ml
Toxocara canis Protein A HRP Conjugate	Blue, Black cap	1 x 20ml