Prestained Protein Ladder

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Overview Product name (AB116029-500UL)

Prestained Protein Ladder – Extra broad molecular weight (5 – 245 kDa)

  • Tested applications

    Suitable for: WBSDS-PAGEmore details
  • General notes

    Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

    This product is also sold by Expedeon, an Abcam company as RunBlue™ TriColor Prestained Protein Ladder: NXA6050. This product was previously called Prism Ultra Protein Ladder (3.5 – 245 kDa).

    This prestained protein ladder is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

    Key product features:

    • Broad range: 3.5-245 kDa
    • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
    • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
    • Sharp bands
    • Recommended loading: ~1.5 – 2.5µl

     

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C short term (1-2 weeks). Store at -20°C.
  • Storage buffer

    pH: 7.5
    Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Western Blot Tools and Reagents
    • Protein ladders
  • Alternative names

    • Molecular weight marker
    • Prism protein ladder
    • SDS PAGE protein ladder prestained

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab116029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.
SDS-PAGE
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

>Datasheet download (AB116029-500UL)<

 


Overview Product name (AB116028-500UL)

Prestained Protein Ladder – Broad molecular weight (10-245 kDa)

  • Tested applications

    Suitable for: SDS-PAGEWBmore details
  • General notes

    Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).

    This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.

    Key product features:

    • Broad range: 10-245 kDa
    • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
    • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
    • Sharp bands
    • Recommended loading: ~1.5 – 2.5µl

     

    This product was previously called Prism Ultra Protein Ladder (10-245 kDa).

    Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Please see notes section.
  • Storage buffer

    pH: 7.5
    Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Western Blot Tools and Reagents
    • Protein ladders
  • Alternative names

    • Molecular weight marker
    • Prism protein ladder
    • SDS PAGE protein ladder prestained

     

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab116028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
WB
Use at an assay dependent concentration.~1.5-2.5 µl per well for general western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

 

>Datasheet Download AB116028-500UL<