RealQ Plus 2x Master Mix Green

RealQ Plus 2x Master Mix Green enables real-time-based DNA amplification with high specificity and efficiency. Real-time PCR is a sensitive and reliable method for gene analysis and DNA quantification. RealQ Plus 2x Master Mix Green is the right choice, when expenses and experiment preparation time might be limited or if you need to quickly analyse many genes.

FEATURES

Reliable master mix for real-time PCR
Based on DNA-binding fluorescent dye detection
Reliable quantification
High specificity, stability, reproducibility and efficiency
Reaction set-up at room temperature
Premixed all-in-one 2x solution

DESCRIPTION

RealQ Plus 2x Master Mix Green is composed of TEMPase Hot Start DNA Polymerase, dNTPs, fluorescent dye and an optimised buffer system. Furthermore, this master mix is available with high, low or without ROX™ensuring optimal performance on most of the commonly used real-time PCR instruments. Just add DNA template and primers.

When the fluorescent Green dye is free in the solution, it emits a very low fluorescent signal. As soon as the dye binds to the double-stranded DNA the signal increases significantly (thousand fold), which makes the fluorescent signal of the dye directly proportional to the amount of amplified dsDNA.

SELECT THE CORRECT ROX LEVEL

Use the Real-time PCR instrument guide to find the correct level of ROX™ reference dye matching your Real-time PCR instrument.

HIGH EFFICIENCY AND SPECIFICITY

To examine the efficiency of the RealQ Plus 2x Master Mix Green, High ROX^TM, a 4-fold dilution series with human gDNA was set up for PAH target (203 bp). Samples were made in triplicates starting with 80 ng down to 80 pg gDNA per well. The results depicted here shows high precision and efficiencies close to 100 %. The identical melt curves show a high specificity of the product and the standard curve shows linear detection range and a high accuracy of the TEMPase Hot Start DNA Polymerase

REPRODUCIBILITY

80 replicates of RealQ Plus 2x Master Mix Green, High ROX™ and 20 ng gDNA, show a standard deviation of only 0.084.

STABILITY AT ROOM TEMPERATURE

Two plates were pre-assembled for qPCR reaction and incubated in darkness at room temperature for 48 and 72 hours. The results show high stability and complete inactivation of the TEMPase before hot start. It allows the scientist to set up the reaction and run the plate several hours later, when convenient.