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HMG-CoA Reductase Activity Assay Kit (Colorimetric)
HMG-CoA Reductase Activity Assay Kit (Colorimetric)
This product is currently out of stock and unavailable.
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 10 min
- Sample type: Purified protein
Overview
Product name
HMG-CoA Reductase Activity Assay Kit (Colorimetric)Detection method
ColorimetricSample type
Purified proteinAssay type
Enzyme activityAssay time
0h 10mProduct overview
HMG-CoA Reductase Activity Assay Kit (Colorimetric) (ab204701) is suitable for measuring activity of purified HMG-CoA reductase or for screening inhibitors/activators of HMG-CoA reductase. It is based on the consumption of NADPH by the enzyme, which can be measured by the decrease of absorbance at OD=340 nm. The limit of detection is below 0.05 mU.
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K588 H mg-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric). K588-100 is the same size as the 100 test size of ab204701.
HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is the rate-controlling enzyme of the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. The enzyme is target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol.
Platform
Microplate reader
Properties
Storage instructions
Store at -20°C. Please refer to protocols.
| Components | Identifier | 100 tests |
|---|---|---|
| HMG-CoA Reductase Assay Buffer |  | — |
| HMG-CoA Reductase |  | — |
| HMG-CoA |  | — |
| NADPH |  | — |
| Inhibitor (Atorvastatin, 10 mM) |  | — |
Research areas
- Cardiovascular Â
- Lipids / Lipoproteins Â
- Lipid Metabolism Â
- Cholesterol Metabolism
- Signal Transduction Â
- Metabolism Â
- Lipid metabolism
- Cardiovascular Â
- Atherosclerosis Â
- Lipoprotein metabolism
- Cancer Â
- Cancer Metabolism Â
- Metabolic signaling pathway Â
- Metabolism of lipids and lipoproteins
- Metabolism Â
- Pathways and Processes Â
- Metabolic signaling pathways Â
- Lipid and lipoprotein metabolism Â
- Lipid metabolism
- Metabolism Â
- Pathways and Processes Â
- Metabolic signaling pathways Â
- Lipid and lipoprotein metabolism Â
- Cholesterol Metabolism
- Metabolism Â
- Pathways and Processes Â
- Metabolic signaling pathways Â
- Lipid and lipoprotein metabolism Â
- Lipoprotein metabolism
- Metabolism Â
- Types of disease Â
- Heart disease
Function
This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis.
Pathway
Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Sequence similarities
Belongs to the HMG-CoA reductase family.
Cellular localization
Endoplasmic reticulum membrane. Peroxisome membrane.
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