Overview Product name (AB116029-500UL)
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Tested applications
Suitable for: WB, SDS-PAGEmore details -
General notes
Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
This product is also sold by Expedeon, an Abcam company as RunBlue™ TriColor Prestained Protein Ladder: NXA6050. This product was previously called Prism Ultra Protein Ladder (3.5 – 245 kDa).
This prestained protein ladder is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Key product features:
- Broad range: 3.5-245 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
- Sharp bands
- Recommended loading: ~1.5 – 2.5µl
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks). Store at -20°C. -
Storage buffer
pH: 7.5
Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol -
Research areas
- Kits/ Lysates/ Other
- Tools and Reagents
- Western Blot Tools and Reagents
- Protein ladders
-
Alternative names
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab116029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.
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|
SDS-PAGE |
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
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>Datasheet download (AB116029-500UL)<
Overview Product name (AB116028-500UL)
Prestained Protein Ladder – Broad molecular weight (10-245 kDa)
-
Tested applications
Suitable for: SDS-PAGE, WBmore details -
General notes
Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).
This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.
Key product features:
- Broad range: 10-245 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
- Sharp bands
- Recommended loading: ~1.5 – 2.5µl
This product was previously called Prism Ultra Protein Ladder (10-245 kDa).
Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
Properties
-
Form
Liquid -
Storage instructions
Please see notes section. -
Storage buffer
pH: 7.5
Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol -
Research areas
- Kits/ Lysates/ Other
- Tools and Reagents
- Western Blot Tools and Reagents
- Protein ladders
-
Alternative names
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab116028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
SDS-PAGE |
Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
|
|
WB |
Use at an assay dependent concentration.~1.5-2.5 µl per well for general western transferring. Apply more for thicker (> 1.5 mm) or larger gel.
|