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Human TGF beta 1 ELISA Kit

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Key features and details

  • Sensitivity: 80 pg/ml
  • Range: 18 pg/ml – 4000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human TGF beta 1 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    SamplenMeanSDCV%
    Overall   < 10%
    Inter-assay
    SamplenMeanSDCV%
    Overall   < 12%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 80 pg/ml

  • Range

    18 pg/ml – 4000 pg/ml

  • Recovery

    96 %

    Sample specific recovery
    Sample typeAverage %Range
    Cell culture supernatant97.8785% – 104%
    Serum94.4682% – 102%
    Plasma95.7893% – 103%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human TGF beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100647) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta 1 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Ab100647 was reformulated on 31st May 2018 with new capture and detector antibodies that allows to increase the sensitivity to human TGF beta 1.


    Please note that as a consequence of this change, the procedure and protocol have slightly changed and we encourage you to review the protocol steps before starting your experiments

  • Notes

    Optimization may be required with urine samples.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components1 x 96 tests
    20X Wash Buffer1 x 25ml
    500X HRP-Streptavidin Concentrate1 x 200µl
    5X Assay Diluent1 x 15ml
    Biotinylated anti-Human TGF beta 12 vials
    Recombinant Human TGF beta 1 Standard (lyophilized)2 vials
    Stop Solution1 x 8ml
    TGF beta 1 Microplate (12 x 8 wells)1 unit
    TMB One-Step Substrate Reagent1 x 12ml

  • Research areas

    • Cardiovascular
      •  
    • Angiogenesis
      •  
    • Growth Factors
      •  
    • TGF
    • Signal Transduction
      •  
    • Growth Factors/Hormones
      •  
    • TGF
    • Stem Cells
      •  
    • Signaling Pathways
      •  
    • TGF beta
      •  
    • Secreted
    • Cancer
      •  
    • Growth factors
      •  
    • TGF
    • Cancer
      •  
    • Invasion/microenvironment
      •  
    • Angiogenesis
      •  
    • Angiogenic growth factors
    • Cancer
      •  
    • Cancer Metabolism
      •  
    • Response to hypoxia
    • Kits/ Lysates/ Other
      •  
    • Kits
      •  
    • ELISA Kits
      •  
    • ELISA Kits
      •  
    • Growth factors and hormones ELISA kits
    • Metabolism
      •  
    • Pathways and Processes
      •  
    • Cofactors, Vitamins / minerals
      •  
    • Co-factors
    • Metabolism
      •  
    • Pathways and Processes
      •  
    • Metabolism processes
      •  
    • Hypoxia

  • Function

    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

  • Tissue specificity

    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.

  • Involvement in disease

    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Post-translational
    modifications

    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.

Protocols

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