HSP70 High Sensitivity ELISA Kit

Abcam’s HSP70 High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of HSP70 in samples from. This assay allows for the quantitative determination of inducible HSP70 from cell lysates and tissue extracts.

A HSP70 mouse monoclonal antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A HSP70 polyclonal antibody is then added, incubated and washed. An HRP conjugated anti-IgG antibody is then added, incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.

Get higher sensitivity in only 90 minutes with Human HSP70 ELISA Kit (ab187399) from our SimpleStep ELISA^® range.

HSP70 is a molecular chaperone whose expression is induced upon exposure of the cell or organism to conditions of stress. It prevents protein aggregation and promotes the refolding of proteins that become damaged in response to environmental insults, pathogens, and disease. Its activity is essential for cellular survival and recovery under stress conditions, as well as for the maintenance of normal cellular function under non-stress conditions. HSP70 has been implicated to play a role in a variety of disease and physiological processes such as hyperthermia, hypertension, toxic exposure to chemical agents, hypoxia, ischemia, inflammation, autoimmunity, apoptosis, cancer, organ transplantation, and bacterial and viral infections. HSP70 is also a key regulator of many normal physiological processes including aging, spermatogenesis, menstruation, and physical activity such as exercise. The HSP70 high sensitivity ELISA kit is designed to evaluate and monitor HSP70 in these processes, providing a key research tool to understand the role of HSP70 in physiology and disease.

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