Human Insulin ELISA Kit

Key features and details

Sensitivity: 4 µlU/ml
Range: 4.69 µlU/ml – 300 µlU/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

Overview

Product name

Human Insulin ELISA Kit
See all Insulin kits

Detection method

Colorimetric

Sample type

Cell culture supernatant, Serum, Plasma

Assay type

Sandwich (quantitative)

Sensitivity

< 4 µlU/ml

Range

4.69 µlU/ml – 300 µlU/ml

Recovery

89 %

Sample specific recovery
Sample type	Average %	Range
Cell culture supernatant	76.16	68% – 88%
Serum	91.4	83% – 102%
Plasma	99.03	73% – 128%

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig

Product overview

Abcam’s Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Insulin and Proinsulin in serum, plasma, cell culture supernatants.

This assay employs an antibody specific for Human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This kit detects both insulin and proinsulin.

Get higher sensitivity in only 90 minutes with Human Insulin ELISA Kit (ab200011) from our SimpleStep ELISA^® range.

Notes

Optimization may be required with urine samples.

Platform

Microplate

Properties

Storage instructions

Store at -20°C. Please refer to protocols.

Components	1 x 96 tests
20X Wash Buffer	1 x 25ml
500X HRP-Streptavidin Concentrate	1 x 200µl
5X Assay Diluent B	1 x 15ml
Assay Diluent A	1 x 30ml
Biotinylated anti-Human Insulin	2 vials
Insulin Microplate (12 strips x 8 wells)	1 unit
Recombinant Human Insulin Standard (lyophilized)	2 vials
Stop Solution	1 x 8ml
TMB One-Step Substrate Reagent	1 x 12ml

Research areas

Signal Transduction
Growth Factors/Hormones
Insulin / Insulin-like

Neuroscience
Neurology process
Metabolism

Signal Transduction
Metabolism
Lipid metabolism

Cancer
Growth factors
Insulin and insulin-like

Cardiovascular
Atherosclerosis
Diabetes associated

Kits/ Lysates/ Other
Kits
ELISA Kits
ELISA Kits
Growth factors and hormones ELISA kits

Metabolism
Types of disease
Diabetes

Metabolism
Types of disease
Obesity

Metabolism
Types of disease
Heart disease

Function

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Involvement in disease

Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.