Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
Overview
Product name
Cholesterol Assay Kit – HDL and LDL/VLDL
See all HDL LDL/VLDL kits
See all HDL LDL/VLDL kits
Detection method
Colorimetric/Fluorometric
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Lysate
Assay type
Quantitative
Assay time
1h 00m
Product overview
Cholesterol Assay Kit ab65390 uses a simple method to quantify total cholesterol, free cholesterol, and cholesterol esters in mammalian samples. It also includes an easy method to separate HDL and LDL / VLDL cholesterol.Â
In the cholesterol assay protocol, cholesterol oxidase acts on free cholesterol to produce a chemical which reacts with a probe to generate color (570 nm) and fluorescence (Ex/Em = 538/587 nm). Cholesterol esterase is used to hydrolyze cholesteryl ester into free cholesterol.
If cholesterol esterase is included in the assay, total cholesterol is measured. If it is not included, free cholesterol is measured. The amount of cholesterol ester can be calculated by subtracting free cholesterol from total cholesterol.
Properties
Storage instructions
Store at -20°C. Please refer to protocols.
Components | Identifier | 100 tests |
---|---|---|
2X LDL/VLDL Precipitation Buffer | NW | 1 x 10ml |
Cholesterol Assay Buffer | WM | 1 x 25ml |
Cholesterol Esterase (Lyophilized) | Blue | 1 vial |
Cholesterol Probe (in DMSO, anhydrous) | Red | 1 x 200µl |
Cholesterol Standard (2 ug/ul) | Yellow | 1 x 100µl |
Enzyme Mix (Lyophilized) | Green | 1 vial |
Research areas
- Cardiovascular Â
- Lipids / Lipoproteins Â
- Lipid Metabolism Â
- Cholesterol Metabolism
- Kits/ Lysates/ Other Â
- Kits Â
- Cell Metabolism Kits Â
- Other Metabolism Assay
- Kits/ Lysates/ Other Â
- Kits Â
- Cell Metabolism Kits Â
- Lipid Metabolism Kits Â
- Cholesterol
- Kits/ Lysates/ Other Â
- Kits Â
- Cell Metabolism Kits Â
- Lipid Metabolism Kits Â
- Obesity
- Metabolism Â
- Pathways and Processes Â
- Metabolic signaling pathways Â
- Lipid and lipoprotein metabolism Â
- Cholesterol Metabolism
- Metabolism Â
- Types of disease Â
- Metabolic disorders
- Metabolism Â
- Pathways and Processes Â
- Redox metabolism Â
- Fatty acid oxidation
Relevance
HDL (high-density-lipoprotein), LDL (low-density lipoprotein) and VLDL (very low-density lipoprotein) are lipoproteins that act as carrier proteins for cholesterol. The regulation of HDL-cholesterol and LDL-cholesterol plays a central role in various disease development. It is well known that low levels of HDL and high level of LDL are associated with an increased risk of cardiovascular events.
Alternative names
- High density lipoprotein
- LDL
- Low density lipoprotein
- Very low density lipoprotein
- VLDL