Native Human IgE protein (Azide free)

Key features and details

Expression system: Native
Purity: > 90% SDS-PAGE
Endotoxin level: < 25.000 Eu/mg
Active: Yes
Suitable for: SDS-PAGE, IHC – Wholemount, WB, ELISA, ICC

Overview

Product name

Native Human IgE protein (Azide free)

Biological activity

1 mg of ab65866 is 380,000± 50,000 IU/m.

Purity

> 90 % SDS-PAGE.
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.

Endotoxin level

< 25.000 Eu/mg

Expression system

Native

Accession

P0DOX4
P0DOX7

Protein length

Full length protein

Animal free

Nature

Native

Amino Acid Sequence 1

Species

Human

Additional sequence information

Observed band of ~185kDa on SDS-PAGE

Description

Native human IgE protein (Azide free)

Properties

Our Abpromise guarantee covers the use of ab65866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

SDS-PAGE

IHC – Wholemount

Western blot

ELISA

Immunocytochemistry

Form

Liquid

Additional notes

This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.

This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.

– Low batch-to-batch variation

– Higher degree of purity (>90%)

– A more uniform product (monoclonal and not polyclonal)

Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cell proliferation studies.