Cell Counting Kit 8 (WST-8 / CCK8)

Cell Counting Kit 8 (WST-8 / CCK8) (ab228554) provides a convenient and robust way of performing a cell viability assay. The kit uses a water-soluble tetrazolium salt to quantify the number of live cells by producing an orange formazan dye upon bio-reduction in the presence of an electron carrier.

WST-8 / CCK8 solution is added directly to the test cells with no pre-mixing of components required.

WST-8 / CCK8 tetrazolium salt is reduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells and is measured by absorbance at 460 nm.

The excellent stability and little cytotoxicity of the WST-8 / CCK8 solution make the kit useful for assays that require long incubation (such as 24 to 48 hours).

The detection sensitivity is higher than with other tetrazolium salt-based assays such as MTT, XTT or MTS etc.

Review the cell health assay guide to learn about other kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.