Human IL-6 ELISA Kit

Key features and details

Sensitivity: 2 pg/ml
Range: 6.25 pg/ml – 200 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

Overview

Product name

Human IL-6 ELISA Kit

Detection method

Colorimetric

Precision

Intra-assay
Sample	n	Mean	SD	CV%
Pooled sampl	15			4.2%

Inter-assay
Sample	n	Mean	SD	CV%
Sample 1	18	200pg/ml	16	8.1%
Sample 2	18	77pg/ml	8	10.1%
Sample 3	18	63pg/ml	6	8.6%
Sample 4	18	103pg/ml	6	6%
Sample 5	18	53pg/ml	3	5.5%

Sample type

Cell culture supernatant, Serum, Plasma

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/ml

Range

6.25 pg/ml – 200 pg/ml

Recovery

96 %

Assay time

1h 45m

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Human

Product overview

Abcam’s IL-6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in Human serum, plasma, buffered solutions or supernatants.

A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.

This kit will recognize both endogenous and recombinant Human IL-6.

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Platform

Microplate

Properties

Storage instructions

Store at +4°C. Please refer to protocols.

Components	Identifier	1 x 96 tests	2 x 96 tests
10X Standard Diluent Buffer	Black	1 x 15ml	1 x 25ml
200X Wash Buffer	White	1 x 10ml	2 x 10ml
Adhesive plate seal		1 x 2 units	1 x 4 units
Biotinylated Antibody Diluent	Red	1 x 7.5ml	1 x 13ml
Biotinylated anti-IL-6	Red	1 x 0.4ml	2 x 0.4ml
Chromogen TMB Substrate Solution		1 x 11ml	1 x 24ml
HRP Diluent	Red	1 x 12ml	1 x 23ml
IL-6 Control	Yellow	2 vials	4 vials
IL-6 Microplate (12 x 8 well strips)		1 unit	2 units
IL-6 Standard (Lyophilized)	Orange	2 vials	4 vials
Standard Diluent (Serum)		1 x 7ml	2 x 7ml
Stop Reagent	Black	1 x 11ml	2 x 11ml
Streptavidin-HRP		2 x 5µl	4 x 5µl

Research areas

Immunology
Innate Immunity
Macrophage / Inflamm.

Immunology
Innate Immunity
Cytokines
Interleukins

Neuroscience
Neurology process
Metabolism

Microbiology
Organism
Virus
RNA Virus
ssRNA positive strand virus
SARS Coronavirus

Cancer
Invasion/microenvironment
Angiogenesis
Angiogenic growth factors

Cancer
Tumor immunology
Cytokines
Interleukins

Kits/ Lysates/ Other
Kits
ELISA Kits
ELISA Kits
Cytokines and cytokine receptors ELISA kits

Metabolism
Types of disease
Obesity

Metabolism
Types of disease
Metabolic disorders

Neuroscience
Processes

Function

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

Involvement in disease

Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.