Thromboxane B2 ELISA Kit

Key features and details

Sensitivity: 10.54 pg/ml
Range: 13.7 pg/ml – 10000 pg/ml
Sample type: Hep Plasma, Saliva, Serum, Tissue Culture Media, Urine
Detection method: Colorimetric
Assay type: Competitive
Reacts with: Human

Overview

Product name

Thromboxane B2 ELISA Kit

Detection method

Colorimetric

Precision

Intra-assay
Sample	n	Mean	CV%
Low	24	759pg/ml	= 3.6%
Medium	24	1283pg/ml	= 4%
High	24	2605pg/ml	= 1.6%

Inter-assay
Sample	n	Mean	CV%
Low	8	44pg/ml	= 7.6%
Medium	8	371pg/ml	= 3.6%
High	8	3053pg/ml	= 6.2%

Sample type

Saliva, Urine, Serum, Tissue Culture Media, Hep Plasma

Assay type

Competitive

Sensitivity

= 10.54 pg/ml

Range

13.7 pg/ml – 10000 pg/ml

Recovery

Sample specific recovery
Sample type	Average %	Range
Saliva	= 98.3	% – %
Urine	= 102.2	% – %
Serum	= 113	% – %
Tissue Culture Media	= 104.7	% – %
Hep Plasma	= 111	% – %

Assay time

3h 00m

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Human
Predicted to work with: a wide range of other species

Product overview

Abcam’s Thromboxane B₂ (TXB₂) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thromboxane B₂ in biological fluids.

A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-TXB₂ antigen and a rabbit polyclonal antibody specific to TXB₂. After incubation the excess reagents are washed away and pNpp substrate is added. After a short incubation the enzyme reaction is stopped and a yellow color is generated. The intensity of the yellow coloration is inversely proportional to the amount of TXB₂ captured in the plate.

Notes

Thromboxane A₂ (TXA₂) is involved in platelet aggregation, vasoconstriction and reproductive functions, but has a half life of only 37 seconds under physiological conditions. Thromboxane B₂ (TXB₂) is the stable product of the non-enzymatic hydration of TXA₂, thus the production of TXA₂ in vivo is typically monitored by measurement of TXB₂ and its metabolite 2,3-dinor TXB₂. 11-dehydro-TXB₂ is a major metabolite of Thromboxane B₂ (TXB₂) found in urine and plasma produced by its dehydrogenation by the enzyme 11-OH-dehydrogenase.

Cross Reactivity

Compound	Cross Reactivity
TXB₂	100%
2,3-dinorTXB₂	7.1%
11-dehydro TXB₂	0.4%
6-keto-PGF_1α	0.23%
PGD₂	<0.1%
PGE₂	<0.1%
PGF_1α	<0.1%
PGF_2α	<0.1%

Platform

Microplate

Properties

Storage instructions

Store at +4°C. Please refer to protocols.

Components	1 x 96 tests
20X Wash Buffer Concentrate	1 x 27ml
Assay Buffer	1 x 27ml
Goat anti-rabbit IgG Microplate (12 x 8 wells)	1 unit
Plate Sealer	1 unit
pNpp Substrate	1 x 20ml
Rabbit Polyclonal Thromboxane B2 Antibody	1 x 5ml
Stop Solution	1 x 5ml
Thromboxane B2 Alkaline Phosphatase Conjugate	1 x 5ml
Thromboxane B2 Standard	1 x 500µl