Urea Assay Kit

Key features and details

Assay type: Quantitative
Detection method: Colorimetric
Platform: Microplate reader
Assay time: 1 hr
Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue Extracts, Urine
Sensitivity: 0.5 nmol/well

Overview

Product name

Urea Assay Kit

Detection method

Colorimetric

Sample type

Cell culture supernatant, Urine, Serum, Plasma, Tissue Extracts, Cell Lysate

Assay type

Quantitative

Sensitivity

> 0.5 nmol/well

Range

0.5 nmol/well – 5 nmol/well

Assay time

1h 00m

Product overview

Urea Assay Kit ab83362 is a rapid, simple, sensitive, and reliable assay used to quantify urea in a variety of samples such as serum, plasma, and urine, etc. It can be used as a blood urea nitrogen assay kit (BUN assay kit).

In the urea assay protocol, urea is acted on by enzymes to form a product that reacts with a probe to generate color (ODmax=570nm). The absorbance is directly proportional to the concentration of urea in the solution. The kit can detect as low as 0.5 nmol per well or 10 µM of urea.

Urea assay protocol summary:
– add samples and standards to wells
– add reaction mix and incubate for 60 min at 37ºC
– analyze with microplate reader

Notes

This product is manufactured by BioVision, an Abcam company and was previously called K375 Urea Colorimetric Assay Kit. K375-100 is the same size as the 100 test size of ab83362.

Platform

Microplate reader

Properties

Storage instructions

Store at -20°C. Please refer to protocols.

Components	Identifier	100 tests	2000 tests
Converting Enzyme (Lyophilised)	Blue	1 vial	20 vials
Developer (Lyophilised)	Orange	1 vial	20 vials
Enzyme Mix (lyophilized)	Green	1 vial	20 vials
OxiRed Probe in DMSO	Red	1 x 200µl	20 x 200µl
Urea Assay Buffer	WM	1 x 25ml	20 x 25ml
Urea Standard (100mM)	Yellow	1 x 100µl	20 x 100µl

Research areas

Kits/ Lysates/ Other
Kits
Cell Metabolism Kits
Amino Acid Assays

Kits/ Lysates/ Other
Kits
Cell Metabolism Kits
Other Metabolism Assay

Relevance

Urea is a waste product which is produced in the liver, dissolved in blood (in a concentration of 2.5 – 7.5 mM), and secreted by the kidneys. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons.