Human ACE2 ELISA Kit

Human ACE2 ELISA Kit (ab235649) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of native ACE2 protein in tissue extracts and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ACE2 with 1052 pg/ml sensitivity.

Note: Plasma, serum, and cell culture media samples were validated with spiked recombinant ACE2. Neat serum from ten individual healthy human female/male donors was measured in duplicate and all values were below the detectable range of the assay.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

        – Single-wash protocol reduces assay time to 90 minutes or less
        – High sensitivity, specificity and reproducibility from superior antibodies
        – Fully validated in biological samples
        – 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ACE2 is a key enzyme in the renin-angiotensin system which regulates blood pressure. Specifically, ACE2 cleaves the terminal nonapeptide from angiotensin I or the terminal septapeptide from angiotensin II to create angiotensin[1-9] and angiotensin[1-7], respectively. The produced peptides work to oppose the effects of angiotensin II and act as a vasodilator. This enzyme is currently under investigation as a potential target for reducing instances of heart failure.

Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.