Key features and details
- Mouse monoclonal [MEM-147] to HLA Class I
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Overview
Product name
Anti-HLA Class I antibody [MEM-147]
See all HLA Class I primary antibodies
See all HLA Class I primary antibodies
Description
Mouse monoclonal [MEM-147] to HLA Class I
Host species
Mouse
Specificity
Reacts with all human classical MHC Class I molecules in native cell-surface forms. Positive in immunoblotting with many (not all) MHC Class I allelic forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). Positive in Western blotting (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32 (ab7855).
Tested applications
Suitable for: Flow Cyt, WBmore details
Species reactivity
Reacts with:Â Human
Immunogen
Tissue, cells or virus corresponding to HLA Class I. PHA-activated peripheral blood lymphocytes
General notes
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Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS
Preservative: 0.097% Sodium azide
Constituent: PBS
Concentration
- 100 µg at 1 mg/ml
Purity
Protein A purified
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE.
Clonality
Monoclonal
Clone number
MEM-147
Isotype
IgG1
Research areas
- Immunology Â
- Adaptive Immunity Â
- MHC Â
- Class I