Pig IgA ELISA Kit

Key features and details

Sensitivity: 4.38 ng/ml
Range: 12.5 ng/ml – 400 ng/ml
Sample type: Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Pig

Overview

Product name

Pig IgA ELISA Kit
See all IgA kits

Detection method

Colorimetric

Precision

Intra-assay
Sample	n	Mean	SD	CV%
Overall				< 10%

Inter-assay
Sample	n	Mean	SD	CV%
Overall				< 10%

Sample type

Serum, Plasma

Assay type

Sandwich (quantitative)

Sensitivity

4.38 ng/ml

Range

12.5 ng/ml – 400 ng/ml

Recovery

Sample specific recovery
Sample type	Average %	Range
Serum	> 85	% – %

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Pig

Product overview

Abcam’s IgA Pig ELISA kit (ab190536) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgA in pig serum and plasma.

In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Platform

Microplate

Properties

Components	1 x 96 tests
20X Wash Buffer Concentrate	1 x 50ml
5X Diluent Concentrate	1 x 50ml
IgA Pig Antibody coated microwells	1 unit
IgA Pig Calibrator (Lyophilized)	1 vial
IgA Pig HRP Conjugate	1 vial
Stop Solution	1 x 12ml
Chromogen Substrate Solution	1 x 12ml

Research areas

Kits/ Lysates/ Other
Kits
ELISA Kits
ELISA Kits
Immunoglobulins ELISA kits

Relevance

Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.