Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)

Key features and details

Assay type: Quantitative
Detection method: Colorimetric/Fluorometric
Platform: Microplate reader
Assay time: 1 hr 20 min
Sample type: Cell culture extracts, Plasma, Tissue Extracts, Urine
Sensitivity: 0.1 nmol/well

Overview

Product name

Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)
See all Lipid Peroxidation kits

Detection method

Colorimetric/Fluorometric

Sample type

Urine, Plasma, Cell culture extracts, Tissue Extracts

Assay type

Quantitative

Sensitivity

> 0.1 nmol/well

Assay time

1h 20m

Product overview

Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970) provides a convenient tool for sensitive detection of malondialdehyde (MDA).

In the lipid peroxidation assay protocol, the MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

Properties

Storage instructions

Store at -20°C. Please refer to protocols.

Components	Identifier	100 tests	2000 tests
BHT	Purple	1 x 1ml	20 x 1ml
MDA Lysis Buffer	WM	1 x 25ml	20 x 25ml
MDA Standard (4.17M)	Yellow	1 x 100µl	20 x 100µl
Phosphotungstic Acid Solution	NM	1 x 12.5ml	20 x 12.5ml
TBA	NM	4 vials	80 vials

Research areas

Signal Transduction
Metabolism
Lipid metabolism

Kits/ Lysates/ Other
Kits
Cell Metabolism Kits
Lipid Metabolism Kits
Obesity

Kits/ Lysates/ Other
Kits
Cell Damage Kits
Oxidative stress

Kits/ Lysates/ Other
Kits
Cell Metabolism Kits
Lipid Metabolism Kits
Lipid metabolism

Metabolism
Pathways and Processes
Redox metabolism
Fatty acid oxidation

Kits/ Lysates/ Other
Kits
Cell Damage Kits
Lipid oxidation

Relevance

Lipid peroxidation refers to the oxidative degradation of lipids and is a well-defined mechanism of cellular damage. The formation of lipid peroxidation products leads to spread of free radical reactions leading to cell damage.