Alkaline Phosphatase Assay Kit

Key features and details

Assay type: Enzyme activity (quantitative)
Detection method: Colorimetric
Platform: Microplate reader
Assay time: 1 hr 10 min
Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Sensitivity: 10 µU

Overview

Product name
Alkaline Phosphatase Assay Kit (Colorimetric)
See all Alkaline phosphatase kits
Detection method
Colorimetric
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
Assay type
Enzyme activity (quantitative)
Sensitivity
> 10 µU
Range
10 µU – 250 µU
Assay time
1h 10m
Species reactivity
Reacts with: Mammals, Other species
Product overview
Alkaline Phosphatase Assay Kit (Colorimetric) ab83369 is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure alkaline phosphatase (ALP) activity in serum and other mammalian samples.

The kit is a pNPP assay; it uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax= 405 nm) when dephosphorylated by ALP.

Alkaline phosphatase assay protocol summary:
– add samples and standards to wells
– add pNPP solution to sample wells (not to standards)
– add ALP enzyme solution to standard wells (not to samples)
– incubate for 60 min at room temp
– add stop solution
– analyze with microplate reader
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K412 Alkaline Phosphatase Activity Colorimetric Assay Kit. K412-500 is the same size as the 500 test size of ab83369.
This kit contains 10 substrate tablets providing convenience for multiple usages.
pNPP Assays
pNPP assays are a class of phosphatase assays using p-nitrophenyl phosphate (pNPP) as a phosphatase substrate. They can be used to measure alkaline phosphatase, neutral phosphatase, and acid phosphatase activity. These different types of phosphatases are active respectively in alkaline, neutral, and acid assay buffers. pNPP assays include Alkaline Phosphatase Assay Kit ab83369 and Acid Phosphatase Assay Kit ab83367.
Platform
Microplate reader

Properties

Storage instructions

Store at -20°C. Please refer to protocols.

Components	Identifier	500 tests	10000 tests
ALP Assay buffer	NM	1 x 100ml	20 x 100ml
ALP Enzyme (Lyophilised)	White	1 vial	20 vials
pNPP Substrate (10 tablets)	Red	1 vial	20 vials
Stop Solution	WM	1 x 10ml	20 x 10ml

Research areas

Signal Transduction
Cytoskeleton / ECM
Extracellular Matrix
Structures
Bone

Kits/ Lysates/ Other
Kits
IHC Kits

Developmental Biology
Organogenesis
Skeletal development
Bone

Kits/ Lysates/ Other
Kits
Cell Signaling Kits
Phosphatase assay kits

Relevance

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone.