ATP Assay Kit (Colorimetric/Fluorometric)

ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) use a robust, simple method; the ATP assay protocol relies on the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (ODmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.

This kit can detect as low as 1 µM of ATP in various samples.

ATP assay protocol summary:
– add samples (deproteinized) and standards to wells
– add reaction mix and incubate for 30 min at room temp
– analyze with microplate reader

Chinese protocol available. See protocols section below.

If you require a more sensitive product, we recommend Luminescent ATP Detection Assay Kit (ab113849), which can detect as low as 1 pM of ATP.

This product is manufactured by BioVision, an Abcam company and was previously called K354 ATP Colorimetric/Fluorometric Assay Kit. K354-100 is the same size as the 100 test size of ab83355.

Related assays

Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay. 

Review the metabolism assay guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

How other researchers have used ATP Assay Kit ab83355

This ATP assay kit has been used in publications in a variety of sample types, including:
– Human: cell culture lysates¹, primary monocyte cell culture lysates², HCT116 cell culture supernatants³
– Mouse: heart tissue⁴, liver⁵, C2C12 and L929 cell lysates⁶, primary thymocyte cell culture lysates⁷, cardiac tissue⁸
– Rat: primary hippocampal neuron cell culture lysates⁹, liver tissue¹⁰, skeletal muscle¹¹
– Pig: kidney cell culture lysates¹², heart tissue¹³ 
– C elegans tissue¹⁴
– Chlamydomonas reinhardtii algae¹⁵

Platform