Pig IL-6 ELISA Kit

Key features and details

Sensitivity: 45 pg/ml
Range: 40.96 pg/ml – 10000 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Pig

Overview

Product name

Pig IL-6 ELISA Kit

Detection method

Colorimetric

Sample type

Cell culture supernatant, Serum, Plasma

Assay type

Sandwich (quantitative)

Sensitivity

< 45 pg/ml

Range

40.96 pg/ml – 10000 pg/ml

Recovery

89 %

Sample specific recovery
Sample type	Average %	Range
Cell culture supernatant	108.3	95% – 117%
Serum	93.47	85% – 102%
Plasma	85.73	67% – 99%

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Pig

Product overview

Abcam’s IL-6 (Interleukin-6) Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-6 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for pig IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Platform

Microplate

Properties

Storage instructions

Store at -20°C. Please refer to protocols.

Research areas

Immunology
Innate Immunity
Macrophage / Inflamm.

Immunology
Innate Immunity
Cytokines
Interleukins

Neuroscience
Neurology process
Metabolism

Microbiology
Organism
Virus
RNA Virus
ssRNA positive strand virus
SARS Coronavirus

Cancer
Invasion/microenvironment
Angiogenesis
Angiogenic growth factors

Cancer
Tumor immunology
Cytokines
Interleukins

Kits/ Lysates/ Other
Kits
ELISA Kits
ELISA Kits
Cytokines and cytokine receptors ELISA kits

Metabolism
Types of disease
Obesity

Metabolism
Types of disease
Metabolic disorders

Neuroscience
Processes

Function

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.