Caspase-3 Assay Kit (Colorimetric)

Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.

The Caspase-3 assay protocol is based on the formation of the chromophore p-nitroaniline (p-NA) by cleavage from the labeled substrate DEVD-pNA. The p-NA can be quantified using a spectrophotometer or a microtiter plate reader reading absorbance at 400 or 405 nm.

Comparison of the absorbance of p-NA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3 activity.

Caspase-3 assay protocol summary:
– add samples to wells
– add reaction buffer and DEVD-p-NA substrate and incubate for 60-120 min at 37ºC
– analyze with microplate reader