Triglyceride Assay Kit – Quantification

Triglyceride Assay Kit (ab65336) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples. In the triglyceride assay protocol, triglycerides are converted to free fatty acids and glycerol. Glycerol is then oxidized to generate a product which reacts with a probe to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).

Triglyceride assay protocol summary: 
– add samples and standards to wells
– add assay buffer and lipase, and incubate for 20 min
– add triglyceride reaction mix and incubate for 60 min
– analyze with microplate reader

Please note: the general range is 0-10 nmol (colorimetric) and 0-1 nmol (fluorometric).

If your sample contains reducing substances, they are likely to interfere with the assay. In this case, we recommend using Triglyceride Assay Kit (Fluorometric, Reducing samples) ab178780.

Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

This product is manufactured by BioVision, an Abcam company and was previously called K622 Triglyceride Quantification Colorimetric/Fluorometric Kit. K622-100 is the same size as the 100 test size of ab65336.

How other researchers have used Triglyceride Assay Kit ab65336

The Triglyceride assay kit has been used in publications in a variety of sample types, including:
– Human: serum¹, plasma², mammary epithelial and mammary cancer cell line lysate³, Huh7.5 hepatocyte-derived cell line lysate⁴, primary liver cell line lysates⁵, sebocyte cell culture lysates⁶
– Mouse: hepatocyte cell lysates⁷, liver extract⁸, serum⁹, plasma¹⁰, kidney extracts¹¹, liver tissue and serum¹², cardiac tissue extracts¹³
– Rat: liver tissue extract¹⁴, plasma¹⁵
– Drosophila¹⁶

Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.