Human BDNF ELISA Kit

Key features and details

One-wash 90 minute protocol
Sensitivity: 2.4 pg/ml
Range: 15.6 pg/ml – 1000 pg/ml
Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

Overview

Product name

Human BDNF ELISA Kit
See all BDNF kits

Detection method

Colorimetric

Precision

Intra-assay
Sample	n	Mean	SD	CV%
Serum	5			2.8%

Inter-assay
Sample	n	Mean	SD	CV%
Serum	3			5.3%

Sample type

Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

Assay type

Sandwich (quantitative)

Sensitivity

2.4 pg/ml

Range

15.6 pg/ml – 1000 pg/ml

Recovery

Sample specific recovery
Sample type	Average %	Range
Cell culture supernatant	106	103% – 109%
Serum	97	94% – 104%
Hep Plasma	103	96% – 112%
EDTA Plasma	98	85% – 108%
Cit plasma	103	95% – 111%

Assay time

1h 30m

Assay duration

One step assay

Species reactivity

Reacts with: Human

Product overview

Human BDNF ELISA Kit (ab212166) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BDNF protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BDNF with 2.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

– Single-wash protocol reduces assay time to 90 minutes or less
– High sensitivity, specificity and reproducibility from superior antibodies
– Fully validated in biological samples
– 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

SPECIES REACTIVITY

This kit recognizes human BDNF protein and is cross-reactive to mouse and rat BDNF protein.

ASSAY SPECIFICITY

This kit recognizes both native and recombinant human BDNF protein in serum, plasma, and cell culture supernatant samples only.

Cell and tissue extract samples have not been tested with this kit.

CROSS REACTIVITY

Recombinant TRKB was prepared at 50 ng/mL and 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.

INTERFERENCE

Recombinant TRKB was prepared at 50 ng/mL and 1 ng/mL and tested for interference with human BDNF at 200 pg/mL. 39% Inference was observed with 1ng/mL and 76% Interference was observed with 50 ng/mL.

Notes

BDNF (Brain derived neurotrophic factor) is a member of the neurotrophin family of growth factors that includes nerve growth factor. BDNF is expressed as a proprotein that is cleaved to form mature BDNF (residues 129-247). This assay measures mature BDNF. Mature BDNF protein sequence is identical between human, mouse, rat and many additional species. BDNF binds to at least 2 receptors: TrkB and LNGFR.

Platform

Pre-coated microplate (12 x 8 well strips)

Properties

Storage instructions

Store at +4°C. Please refer to protocols.

Components	1 x 96 tests
10X Human BDNF Capture Antibody	1 x 600µl
10X Human BDNF Detector Antibody	1 x 600µl
10X Wash Buffer PT (ab206977)	1 x 20ml
Antibody Diluent CPI – HAMA Blocker (ab193969)	1 x 6ml
Human BDNF Lyophilized Recombinant Protein	2 vials
Plate Seals	1 unit
Sample Diluent NS (ab193972)	1 x 50ml
SimpleStep Pre-Coated 96-Well Microplate (ab206978)	1 unit
Stop Solution	1 x 12ml
TMB Development Solution	1 x 12ml

Research areas

Neuroscience
Neurology process
Growth and Development
Neurotrophins

Neuroscience
Neurology process
Neurodegenerative disease
Huntington’s disease

Neuroscience
Neurology process
Neurodegenerative disease
Alzheimer’s disease
Other

Cardiovascular
Atherosclerosis
Diabetes associated

Cardiovascular
Atherosclerosis
Thrombosis
Other

Metabolism
Types of disease
Diabetes

Metabolism
Types of disease
Obesity

Neuroscience
Development

Neuroscience
Processes

Function

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Tissue specificity

Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.