Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
Product name
Detection method
Precision
Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Pos. Serum | 8 | Â | Â | 3.9% |
Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Pos. Serum | 4 | Â | Â | 5.2% |
Sample type
Assay type
Assay duration
Species reactivity
Product overview
Abcam’s anti-Trichinella spiralis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Trichinella spiralis in Human serum and plasma.
A 96-well plate has been precoated with Trichinella spiralis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Trichinella spiralis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Trichinella spiralis IgG sample captured in plate.
Platform
Properties
Storage instructions
Components | Identifier | 1 x 96 tests |
---|---|---|
20X Washing Solution | White cap | 1 x 50ml |
Cover foil | Â | 1 unit |
IgG Sample Diluent | Colored yellow; White cap | 1 x 100ml |
Stop Solution | red cap | 1 x 15ml |
Strip holder | Â | 1 unit |
TMB Substrate Solution | Yellow cap | 1 x 15ml |
Trichinella spiralis (IgG) Coated Microplate (12 x 8 wells) | 12 strips of 8 wells | 1 unit |
Trichinella spiralis IgG Cut-off Control | Colored yellow; Green cap | 1 x 3ml |
Trichinella spiralis IgG Negative Control | Colored yellow; Blue cap | 1 x 2ml |
Trichinella spiralis IgG Positive Control | Colored yellow; Red cap | 1 x 2ml |
Trichinella spiralis Protein A HRP Conjugate | Colored blue; Black cap | 1 x 20ml |
Research areas
- Immunology Â
- Immunoglobulins Â
- Heavy Chain Â
- IgG
- Microbiology Â
- Organism Â
- Eukaryotic Microorganism Â
- Metazoa Â
- Nematoda
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