AGE (Advanced Glycation End) Assay Kit

Key features and details

Assay type: Competitive
Detection method: Colorimetric
Platform: Microplate reader
Sample type: Cell Lysate, Plasma, Purified protein, Serum, Tissue Lysate
Sensitivity: 0.5 µg/ml

Overview

Product name
AGE (Advanced Glycation End) Assay Kit
See all AGE kits
Detection method
Colorimetric
Sample type
Serum, Plasma, Cell Lysate, Purified protein, Tissue Lysate
Assay type
Competitive
Sensitivity
0.5 µg/ml
Range
0.36 µg/ml – 100 µg/ml
Product overview
AGE (Advanced Glycation End Products) Assay Kit (ab238539) is designed for the rapid detection and quantitation of advanced glycation end product protein adducts.

Advanced Glycation End Products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent advanced glycation end products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The quantity of AGE adduct in protein samples is determined by comparing its OD with that of a known AGE-BSA standard curve.
Platform
Microplate reader

Properties

Storage instructions

Please refer to protocols.

Components	96 tests
100X Conjugate Diluent	1 x 300µl
10X Wash Buffer	1 x 100ml
AGE Conjugate	1 x 50µl
AGE-BSA Standard	1 x 125µl
Anti-AGE Antibody (1000X)	1 x 10µl
Assay Diluent	1 x 50ml
Protein Binding Strip Well Plate	1 unit
Secondary Antibody, HRP Conjugate (1000X)	1 x 20µl
Stop Solution	1 x 12ml
Substrate Solution	1 x 12ml

Research areas

Immunology
Immune System Diseases
Autoimmune

Signal Transduction
Metabolism
Energy Metabolism

Cardiovascular
Atherosclerosis
Lipoprotein metabolism

Cardiovascular
Atherosclerosis
Diabetes associated

Cancer
Cancer Metabolism
Metabolic signaling pathway
Metabolism of lipids and lipoproteins

Cancer
Cancer Metabolism
Metabolic signaling pathway
Integration of energy metabolism

Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Lipid metabolism

Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Lipoprotein metabolism

Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Energy Metabolism

Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Integration of energy

Metabolism
Types of disease
Diabetes

Metabolism
Types of disease
Heart disease

Relevance

The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure.