Soluble Collagen Assay Kit

Soluble Collagen Assay Kit (ab241015) detects newly synthesized (acid-soluble) collagen levels in various biological samples and cultured cell medium.

The assay involves extraction of soluble collagen in acetic acid, followed by enzymatic degradation of collagen into glycine-rich oligopeptides, which are quantified using a fluorogenic reagent and developer solution that selectively react with the N-terminal glycine fragments to form a stable fluorescent complex (Ex/Em = 376/468 nm).

The assay is more sensitive and selective than dye-binding (Picrosirius Red) soluble collagen assays, is simple to perform and has a linear range from 0.05 – 2 μg collagen per well (2.5 μg/mL to 100 μg/mL in a 20 μL sample volume).

This product is manufactured by BioVision, an Abcam company and was previously called K532 Soluble Collagen Assay Kit (Fluorometric). K532-100 is the same size as the 100 test size of ab241015.