Mouse CD23 ELISA Kit

Key features and details

One-wash 90 minute protocol
Sensitivity: 2 pg/ml
Range: 31.25 pg/ml – 2000 pg/ml
Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Mouse

Overview

Product name

Mouse CD23 ELISA Kit

Detection method

Colorimetric

Precision

Intra-assay
Sample	n	Mean	SD	CV%
Serum	5			7.9%

Inter-assay
Sample	n	Mean	SD	CV%
Serum	3			9.5%

Sample type

Cell culture supernatant, Serum, EDTA Plasma, Cit plasma

Assay type

Sandwich (quantitative)

Sensitivity

2 pg/ml

Range

31.25 pg/ml – 2000 pg/ml

Recovery

Sample specific recovery
Sample type	Average %	Range
Serum	126.1	119.2% – 133.4%
Cell culture media	120.7	117% – 123.3%
EDTA Plasma	89.2	85.8% – 92.3%
Cit plasma	81.3	77.8% – 83.9%

Assay time

1h 30m

Assay duration

One step assay

Species reactivity

Reacts with: Mouse
Does not react with: Pig

Product overview

Mouse CD23 ELISA Kit (ab206984) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD23 protein in cell culture supernatant, serum, cit plasma, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD23 with 2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

– Single-wash protocol reduces assay time to 90 minutes or less
– High sensitivity, specificity and reproducibility from superior antibodies
– Fully validated in biological samples
– 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Notes

CD23 is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. It has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). It is a 47 kDa type II transmembrane glycoprotein containing a short N-terminal cytoplasmic tail, a transmembrane domain, and extracellular domain containing C-type lectin domain. Two membrane isoforms and soluble CD23 fragements exist.