Catalase Activity Assay Kit (Colorimetric/Fluorometric)

Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.

In the catalase activity assay protocol, the catalase present in the sample reacts with hydrogen peroxide (H₂O₂) to produce water and oxygen. The unconverted H₂O₂ reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 535/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 µU of catalase activity.

Catalase activity assay protocol summary:
– add samples and standards to wells
– add stop solution into sample control wells and incubate at 25ºC for 5 min
– add H2O2 solution into wells and incubate for 30 min at 25ºC
– add stop solution
– add developer mix and incubate for 10 min at 25ºC
– analyze with microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K773 Catalase Activity Colorimetric/Fluorometric Assay Kit. K773-100 is the same size as the 100 test size of ab83464.