TNF-α ELISA Kit allows for the in vitro quantitative determination of TNFα concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.906-250pg/ml
Sensitivity
3.906-250pg/ml
Species
UniProt ID
Mouse
P06804
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TNFα and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
96
EDTA plasma(n=5)
85-105
96
heparin plasma(n=5)
85-105
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.