Ascorbic Acid Assay Kit (Biological Samples)

Key features and details

Assay type: Quantitative
Detection method: Colorimetric
Platform: Microplate reader
Assay time: 30 min
Sample type: Cell culture extracts, Cell culture media, Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Sensitivity: 0.2 nmol/well

Overview

Product name

Ascorbic Acid Assay Kit (Biological Samples)

Detection method

Colorimetric

Sample type

Cell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts, Cell culture media

Assay type

Quantitative

Sensitivity

> 0.2 nmol/well

Range

0.2 nmol/well – 20 nmol/well

Assay time

0h 30m

Product overview

Ascorbic Acid Assay Kit (Biological Samples) (ab65656) provides a rapid, simple, and sensitive means of detecting ascorbic acid (vitamin C) in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products.

In the ascorbic acid assay protocol, Fe3+ is reduced to Fe2+ by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between OD=545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content.

The assay can detect 0.2 to 20 nmol of ascorbic acid.

Ascorbic acid assay protocol summary:
– add samples and standards to wells
– add reaction mix
– analyze with a microplate reader in kinetic mode every minute for 2-3 minutes

Notes

This product is manufactured by BioVision, an Abcam company and was previously called K671 Ascorbic Acid Colorimetric Assay Kit II (FRASC). K671-100 is the same size as the 100 test size of ab65656.

Platform

Microplate reader

Properties

Storage instructions

Store at -20°C. Please refer to protocols.

Components	100 tests
Ascorbate Oxidase (lyophilized)	1 vial
Ascorbic Acid Probe	1 x 1ml
Ascorbic Acid Standard (Lyophilised)	1 vial
FeCl3 solution	1 x 1ml
Ferric Reducing Ascorbate Buffer	1 x 25ml

Research areas

Signal Transduction
Metabolism
Vitamins / Minerals

Cell Biology
Other Antibodies
Oxidative Stress

Kits/ Lysates/ Other
Kits
Cell Damage Kits
Oxidative stress

Kits/ Lysates/ Other
Kits
Cell Damage Kits
Cell Damage

Relevance

Ascorbic Acid (C₆H₈O₆) is an essential nutrient in human diets, and is necessary to maintain connective tissue and bone. The biologically active form of ascorbic acid is vitamic C, a water soluble vitamin which is the L-enantiomer form of ascorbic acid. Humans have lost the ability to synthesize ascorbic acid and must obtain in the diet. Ascorbic acid is an electron donor for enzymes involved in collagen hydroxylation, biosynthesis of carnitine and norepinephrine, tyrosine metabolism and amidatin of peptide hormones. The ability of vitamin C to donate electrons also makes it a potent water-soluble anti-oxidant that readily scavenges free radicals.