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Human Granzyme B ELISA Kit

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Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 106 pg/ml
  • Range: 156 pg/ml – 10000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Granzyme B ELISA Kit
    See all Granzyme B kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    SamplenMeanSDCV%
    Supernatant5  8.5%
    Inter-assay
    SamplenMeanSDCV%
    Supernatant3  10.3%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    106 pg/ml

  • Range

    156 pg/ml – 10000 pg/ml

  • Recovery

     

    Sample specific recovery
    Sample typeAverage %Range
    Cell culture supernatant110100% – 124%
    Serum8987% – 94%
    Hep Plasma9590% – 99%
    Cit plasma9794% – 99%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Granzyme B ELISA Kit (ab235635) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Granzyme B protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Granzyme B with 106 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            – Single-wash protocol reduces assay time to 90 minutes or less
            – High sensitivity, specificity and reproducibility from superior antibodies
            – Fully validated in biological samples
            – 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components1 x 96 tests
    10X Human Granzyme B Capture Antibody1 x 600µl
    10X Human Granzyme B Detector Antibody1 x 600µl
    10X Wash Buffer PT (ab206977)1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971)1 x 1ml
    Antibody Diluent CPI – HAMA Blocker (ab193969)1 x 6ml
    Human Granzyme B Lyophilized Recombinant Protein2 vials
    Plate Seals1 unit
    Sample Diluent NS (ab193972)1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978)1 unit
    Stop Solution1 x 12ml
    TMB Development Solution1 x 12ml

  • Research areas

    • Cell Biology
      •  
    • Apoptosis
      •  
    • Extracellular Signals
      •  
    • Granzymes
    • Immunology
      •  
    • Adaptive Immunity
      •  
    • T Cells
      •  
    • Cytotoxic Cells
    • Immunology
      •  
    • Adaptive Immunity
      •  
    • Regulatory T Cells
    • Immunology
      •  
    • Innate Immunity
      •  
    • NK Cells
    • Neuroscience
      •  
    • Processes

  • Function

    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

  • Sequence similarities

    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.

  • Cellular localization

    Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.

    Alternative names

    • C11
    • Cathepsin G like 1
    • Cathepsin G-like 1
    • CCPI
    • CGL 1
    • CGL1
    • CSP B
    • CSPB
    • CTLA 1
    • CTLA-1
    • CTLA1
    • CTSGL1
    • Cytotoxic serine protease B
    • Cytotoxic T lymphocyte associated serine esterase 1
    • Cytotoxic T lymphocyte proteinase 2
    • Cytotoxic T-lymphocyte proteinase 2
    • Fragmentin 2
    • Fragmentin-2
    • GRAB_HUMAN
    • Granzyme 2
    • Granzyme B
    • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1)
    • Granzyme-2
    • GranzymeB
    • GRB
    • Gzmb
    • Hlp
    • Human lymphocyte protein
    • Lymphocyte protease
    • Protease, serine, B
    • SECT
    • T cell serine protease 1 3E
    • T cell serine protease 1-3E
    • T-cell serine protease 1-3E

Protocols

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